Journal: Molecules

Article Title: Effects of Roxadustat on Erythropoietin Production in the Rat Body

doi: 10.3390/molecules27031119

Figure Lengend Snippet: Effects of ROX on Epo mRNA and protein expression in the kidneys and liver. Effects of ROX on Epo ( a ), HIF2α ( b ), HIF1α ( c ) and PHD2 ( d ) mRNA expressions. ( e ) Effects of ROX on Epo protein expressions in the kidney (left half) and the liver (right half). * indicates p < 0.05. n = 3–9. CK, R5K and R10K show the control, R5- and R10-treated rat kidney, respectively. CL, R5L and R10L show the control, R5- and R10-treated rat liver, respectively.

Article Snippet: The ROX-group rats were given an intraperitoneal injection of ROX (FG-4592; MedChemExpress, Monmouth Junction, NJ, USA at 50 or 100 mg/kg body weight (R5 and R10, respectively), dissolved in 5% glucose with 32.5 mmol/L NaOH) [ ].

Techniques: Expressing, Control

Journal: Molecules

Article Title: Effects of Roxadustat on Erythropoietin Production in the Rat Body

doi: 10.3390/molecules27031119

Figure Lengend Snippet: Immunohistochemical analysis of the effects of ROX on Epo production by the kidneys. Under control conditions, Epo staining was found from the proximal tubule to the collecting duct ( a ). ROX increased Epo production in the peritubular cells around proximal tubules after 6 h (R10 > R5). Red arrowheads show Epo positive peritubular cells (( d – f ) and red square of ( a – c )). Epo staining was not increased in the distal tubules but slightly increased in the proximal tubules by ROX (( g – i ) and yellow square of ( a – c )). Scale bar: 20 µm ( a – c ) and 10 µm ( d – i ). PCT, proximal convoluted tubules; and CNT, connecting tubules.

Article Snippet: The ROX-group rats were given an intraperitoneal injection of ROX (FG-4592; MedChemExpress, Monmouth Junction, NJ, USA at 50 or 100 mg/kg body weight (R5 and R10, respectively), dissolved in 5% glucose with 32.5 mmol/L NaOH) [ ].

Techniques: Immunohistochemical staining, Control, Staining

Journal: Molecules

Article Title: Effects of Roxadustat on Erythropoietin Production in the Rat Body

doi: 10.3390/molecules27031119

Figure Lengend Snippet: Plasma Epo concentration and Western blot analysis of serum Epo. ( a ): Plasma Epo concentration in control and ROX (50 mg/kg)-injected rats. ROX significantly increased the plasma Epo concentration from 1.2 ± 0.1 to 1072 ± 333 mIU/mL (n = 5–6, p < 0.001). ( b ): Serum Epo was examined by Western blot analysis as described in the methods. Plasma Epo concentration in control, R5 (50 mg/kg) and R10 (100 mg/kg) rats was 1.4, 2696 and 3200 mIU/mL, respectively. Glycosylated Epo (Epo) and deglycosylated Epo (degEpo) were detected at 35–38 and 22 kDa, respectively.

Article Snippet: The ROX-group rats were given an intraperitoneal injection of ROX (FG-4592; MedChemExpress, Monmouth Junction, NJ, USA at 50 or 100 mg/kg body weight (R5 and R10, respectively), dissolved in 5% glucose with 32.5 mmol/L NaOH) [ ].

Techniques: Concentration Assay, Western Blot, Control, Injection